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Ovine Insulin EIA羊胰島素檢測(cè)試劑盒背景介紹：

Mercodia Ovine Insulin ELISA provides a method for the quantitative determination of insulin in ovine serum and plasma.

Ovine Insulin EIA羊胰島素檢測(cè)試劑盒SUMMARY AND EXPLANATION OF THE TEST

Insulin is the principal hormone responsible for the control of glucose metabolism. It issynthesised in the ?-cells of the islets of Langerhans as the precursor, proinsulin, which is processed to form C-peptide and insulin. Both are secreted in equimolar amounts into the portalcirculation. The mature insulin molecule comprises two polypeptide chains, the A chain and theB chain. The two chains are linked together by two inter-chain disulphide bridges. There is alsoan intra-chain disulphide bridge in the A chain. Secretion of insulin is mainly controlled by plasma glucose concentration, and the hormonehas a number of important metabolic actions. Its principal function is to control the uptakeand utilisation of glucose in peripheral tissues via the glucose transporter. This and other hypoglycaemic activities, such as the inhibition of hepatic gluconeogenesis and glycogenolysis arecounteracted by the hyperglycaemic hormones including glucagon, epinephrine (adrenaline),growth hormone and cortisol. Extensive research on how to improve the nutritional, metabolic and health status ofruminants has been at focus for a long time. A decrease in dry matter intake is a major physiological change in ruminants. This may lead to several metabolic disorders such as ketosis, fattyliver and hypocalcemia (1-2). Food intake is a complex mechanism, regulated by several factorsincluding for example hormones, metabolites and environmental factors (1-3).

Ovine Insulin EIA羊胰島素檢測(cè)試劑盒PRINCIPLE OF THE PROCEDURE

Mercodia Ovine Insulin ELISA is a solid phase two-site enzyme immunoassay. It is based on thedirect sandwich technique in which two monoclonal antibodies are directed against separateantigenic determinants on the insulin molecule. During incubation, insulin in the samplereacts with peroxidase-conjugated anti-insulin antibodies and anti-insulin antibodies boundto the microtitration well. After a simple washing step that removes unbound enzyme labelledantibody, the bound conjugate is detected by reaction with 3,3′-5,5′-tetramethylbenzidine(TMB). The reaction is stopped by the addition of acid, giving a colorimetric endpoint that canbe read spectrophotometrically.